This video was taken this morning in a cloning lab. My genetics class was taking a tour and they let us inject some oocytes with somatic cells! This is one of my classmates doing an excellent job! She even fixes a mistake! (This is how clones are created!)

Stained oocyte cumulus complex traveling over the surface of a hamster oviduct. This oocyte cumulus complex adheres to the surface of the oviduct and becomes stretched out as the beating cilia pull it over the surface towards the ostium. When it reaches the ostium, it turns the corner and enters.

Our center leads the region in clinical reproductive research and is committed to continued advancement in the field. This video explores research on oocyte cryopreservation by the GRS scientific team, led by Michael J. Tucker, Ph.D. Georgia Reproductive Specialists provides the latest in assisted reproductive technologies such as in vitro fertilization (IVF), blastocyst transfer, egg donation, embryo cryopreservation, ovulation induction and insemination to couples with infertility problems due to endometriosis, polycystic ovary syndrome (PCOS), male factor, fallopian tube obstruction, uterine fibroids and age related issues. GRS offers convenienently located offices in Alpharetta, Northside and Decatur. For more information visit us online at www.ivf.com, call 404-843-2229 or simply attend a free educational seminar "IVF AFTER HOURS" held monthly at our Northside office.

Mader Human Biology Chapter 16: Reproduction

Video showing the importance of adhesion in transfer of the oocyte cumulus complex into the opening of the oviduct. During normal pickup of the oocyte cumulus complex by the oviduct, the matrix of the complex adheres to the tips of the cilia. This adhesion is vital for pickup. In this movie the oocyte cumulus complex had previously been picked up and compressed by the ostium of the oviduct. Compression of the matrix compromises the adhesion of the oocyte cumulus complex to the cilia. This oocyte cumulus complex adheres well enough to travel along the edge of the oviduct but does not adhere well enough to turn the corner and enter the opening of the oviduct.

Birds eye view of the opening of the oviduct showing entry of a stained oocyte cumulus complex. The oocyte undergoes "churning" as it enters the oviduct and the oocyte become displaced to the side of the complex.

Oocyte goes to school to get through ovulation

normaly enucleation for oocyte is squeeze or aspiration method but both method is physical method . if using slit through X-ray oocyte (meta phase plate ) 15- 20 micro meter , oocyte enucleation possible. but not give to X-ray whole oocyte . that make mitochondria lysis. so... 19kPv, 6 mA, 2-9 second through slit X-ray can enucleation possible . that method mitochondria can maintain healthy. conculation : X-ray through slit enuclation is effiency method for oocyte enucleation. and it can easily reach to Bl-stage oocyte. 난자핵의 탈핵을 쉽게 하기위해 엑스레이 x-RAY 를 난자전체에 가하는것이 아니고 15-20 MICRO METER 슬릿을 통해서 난자핵에만 정확히 가함으로 짧은시간에 외형적변화가 없이 정확히 난자핵을 탈핵하여 좀더 높은 효율로 배반포를 얻어 줄기세포주 성립효율을 높이는 방법

High magnification video showing movement of an oocyte cumulus complex into the opening of the oviduct. Cilia can be seen beating at the edge of the opening to the oviduct. The oocyte cumulus complex rides on the surface of the beating cilia, which drag it into the oviduct.

Lycopodium spores being picked up by currents above hamster oviductal cilia. These spores are small particles that do not adhere to the oviduct. They move over the surface of the oviduct in the current created by the cilia.

Mammalian cortical granules in a mouse oocyte

OPU oocyte egg granulosa cell-2

OPU oocyte egg granulosa cell

卵子有放射冠漂亮的卵子,女人生育最重要的就是卵子的品質.卵子的數目,如果取卵出來卵子旁邊有顆粒細胞排列像太陽光芒般是最好的卵

從幹細胞來製造人造卵子，取卵出來人類的卵子長什麼樣子呢?

automatic birth control common spirochete reaches oocyte first It occured to me that birth control could be automatic visualize an oocyte after meeting a sperm it blocks other sperm If the tiny thing that started the oocytes sperm blocking chemistry were a motile bacteria like a spirochete then the vaginal bacteria would reach the egg first passivating the oocyte blocking pregnancy motile chemotactic bacteria or common breeding these bacteria to seek the surface chemistry of an oocyte is rapid as hundreds of billions of bacteria are at every petri dish; just add mutagens to create an oocyte seeking spirochete a drug specific to the bacteria could then remove the spirochete permitting conception; people do their trhing yet children are always conceived on purpose thus a nation with a one child policy could have a widely distributed bacteria that prevented millions or hundreds of millions of nonplanned pregnancies Nations vary, If a nation gave a 3000$ bonus to persons choosing to parent above the age of 23 or even 27 the quality of the parenting as well as the living knowledge of the parents would be greater; Freakonomics suggests crime was reduced as a result of abortion with a 23 or 27 year age of parenting crime would be much reduced as well as social opportunity grown If China with it one child policy truly went from 1.4 billion to 700 million the eventual resource saving rival that of an entire Unite States thus an automatic bacteria terminates pregnancy is currently valued as well as environmentally beneficial notably this bacterial genome if attached to a virus could create recurring automatic presence always preventing unplanned pregnancy

許多卵子..

automatic birth control common spirochete reaches oocyte first It occured to me that birth control could be automatic visualize an oocyte after meeting a sperm it blocks other sperm If the tiny thing that started the oocytes sperm blocking chemistry were a motile bacteria like a spirochete then the vaginal bacteria would reach the egg first passivating the oocyte blocking pregnancy motile chemotactic bacteria or common breeding these bacteria to seek the surface chemistry of an oocyte is rapid as hundreds of billions of bacteria are at every petri dish; just add mutagens to create an oocyte seeking spirochete a drug specific to the bacteria could then remove the spirochete permitting conception; people do their trhing yet children are always conceived on purpose thus a nation with a one child policy could have a widely distributed bacteria that prevented millions or hundreds of millions of nonplanned pregnancies Nations vary, If a nation gave a 3000$ bonus to persons choosing to parent above the age of 23 or even 27 the quality of the parenting as well as the living knowledge of the parents would be greater; Freakonomics suggests crime was reduced as a result of abortion with a 23 or 27 year age of parenting crime would be much reduced as well as social opportunity grown If China with it one child policy truly went from 1.4 billion to 700 million the eventual resource saving rival that of an entire Unite States thus an automatic bacteria terminates pregnancy is currently valued as well as environmentally beneficial notably this bacterial genome if attached to a virus could create recurring automatic presence always preventing unplanned pregnancy

Pair of movies showing the effect of cigarette smoke on oocyte cumulus complex pickup by the oviduct. The oviduct on the left was exposed to sidestream smoke, while the one on the right was incubated in a control medium. Both videos represent ten seconds of recording and show that smoke treatment retards oocyte cumulus complex pickup.

http://www.myspace.com/HumanLifeAwareness http://HumanLifeAwareness.org SOMEONE GET HILLARY AN EMBRYOLOGY TEXTBOOK! EMBRYOLOGISTS ARE UNANIMOUS THAT IT IS AN OBSERVABLE SCIENTIFIC FACT THAT HUMAN LIFE BEGINS AT CONCEPTION/FERTILIZATION. HERE ARE JUST A FEW EXAMPLES: The Developing Human: Clinically Oriented Embryology, 6th ed. Keith L. Moore, Ph.D. & T.V.N. Persaud, Md., (Philadelphia: W.B. Saunders Company, 1998), 2-18: "[The Zygote] results from the union of an oocyte and a sperm. A zygote is the beginning of a new human being. Human development begins at fertilization, the process during which a male gamete or sperm ... unites with a female gamete or oocyte ... to form a single cell called a zygote. This highly specialized, totipotent cell marks the beginning of each of us as a unique individual." From Human Embryology & Teratology, Ronan R. O'Rahilly, Fabiola Muller, (New York: Wiley-Liss, 1996), 5-55. "Fertilization is an important landmark because, under ordinary circumstances, a new, genetically distinct human organism is thereby formed... Fertilization is the procession of events that begins when a spermatozoon makes contact with a secondary oocyte or its investments... The zygote ... is a unicellular embryo.." Essentials of Human Embryology, William J. Larsen, (New York: Churchill Livingstone, 1998), 1-17. "In this text, we begin our description of the developing human with the formation and differentiation of the male and female sex cells or gametes, which will unite at fertilization to initiate the embryonic development of a new individual. ... Fertilization takes place in the oviduct ... resulting in the formation of a zygote containing a single diploid nucleus. Embryonic development is considered to begin at this point... This moment of zygote formation may be taken as the beginning or zero time point of embryonic development." Kaluger, G., and Kaluger, M., Human Development: The Span of Life, page 28-29, The C.V. Mosby Co., St. Louis, 1974 "In that fraction of a second when the chromosomes form pairs, the sex of the new child will be determined, hereditary characteristics received from each parent will be set, and a new life will have begun." "Embryo: The developing individual between the union of the germ cells and the completion of the organs which characterize its body when it becomes a separate organism.... At the moment the sperm cell of the human male meets the ovum of the female and the union results in a fertilized ovum (zygote), a new life has begun.... The term embryo covers the several stages of early development from conception to the ninth or tenth week of life." Clark, J. ed., The Nervous System: Circuits of Communication in the Human Body, page 99, Torstar Books Inc., Toronto, 1985 "Each human begins life as a combination of two cells, a female ovum and a much smaller male sperm. This tiny unit, no bigger than a period on this page, contains all the information needed to enable it to grow into the complex structure of the human body. The mother has only to provide nutrition and protection." The Biology of Prenatal Develpment, National Geographic, 2006. (Video) "Biologically speaking, human development begins at fertilization." Dr. Jerome Lejeune, a famous geneticist, testified in Davis v. Davis, Circuit Court, State of Tennessee, 1989: "...each of us has a unique beginning, the moment of conception...As soon as the twenty-three chromosomes carried by the sperm encounter the twenty-three chromosomes carried by the ovum, the whole information necessary and sufficient to spell out all the characteristics of the new being is gathered...(W)hen this information carried by the sperm and by the ovum has encountered each other, then a new human being is defined which has never occurred before and will never occur again...[the zygote, and the cells produced in the succeeding divisions] is not just simply a non-descript cell, or a "population" or loose "collection" of cells, but a very specialized individual, i.e., someone who will build himself according to his own rule." (As quoted in Linacre Quarterly, February, 1993) * "Father of Modern Genetics" Dr. Jerome Lejeune told the lawmakers: "To accept the fact that after fertilization has taken place a new human has come into being is no longer a matter of taste or opinion ... it is plain experimental evidence."

Part one of our assisted reproduction techniques video shows the process of egg retrieval. Transvaginal oocyte collection is a procedure designed to remove eggs from ovaries. San Diego Fertility Center is staffed with infertility specialists who are among the top fertility doctors in the nation.

Josh: We recorded this the day after Nancy Pelosi made her controversial statements. I have never become so frustrated during the recording of a show, and it was hours later before my heart rate got back down to normal. I'm uploading this way before the full episode will go live at ProLifePodcast.net because this is happening now, and I don't want to wait a month before we get this out. The entire show was awesome, and I'm looking forward to you having the chance to hear it. This video is from the 32nd episode of Life Report. Listen to past shows at http://ProLifePodcast.net. Be sure to SUBSCRIBE to our channel! EVIDENCE THAT LIFE BEGINS AT FERTILIZATION: - "[The Zygote] results from the union of an oocyte and a sperm. A zygote is the beginning of a new human being. Human development begins at fertilization, the process during which a male gamete or sperm ... unites with a female gamete or oocyte ... to form a single cell called a zygote. This highly specialized, totipotent cell marks the beginning of each of us as a unique individual." (The Developing Human: Clinically Oriented Embryology, 6th ed. Keith L. Moore, Ph.D. & T.V.N. Persaud, Md., (Philadelphia: W.B. Saunders Company, 1998), 2-18.) - • "It is the penetration of the ovum by a spermatozoan and resultant mingling of the nuclear material each brings to the union that constitutes the culmination of the process of fertilization and marks the initiation of the life of a new individual."( Human Embryology, 3rd ed. Bradley M. Patten, (New York: McGraw Hill, 1968), 43.) - • "The zygote thus formed represents the beginning of a new life." (Briological Principles and Modern Practice of Obstetrics. J.P. Greenhill and E.A. Friedman, (Philadelphia: W.B. Sanders, 1974), 17.) - • "Every time a sperm cell and ovum unite a new being is created which is alive and will continue to live unless its death is brought about by some specific condition." (Pathology of the Fetus and the Infant, 3d ed. E.L. Potter and J.M. Craig, (Chicago: Year Book Medical Publishers, 1975), vii.) - Former president of Planned Parenthood Alan Guttmacher said that life begins at conception, and that "this all seems so simple and evident to us that it is difficult to picture a time when it was not part of the common knowledge." (Life in the Making, Viking Press, 1933.) - "Conception confers life..." Rites of Life: The Scientific Evidence for Life Before Birth, by Landrum B Shettles, Zondervan (December 1983) - After eight days of hearing consisting of 57 witnesses, including world-renowned geneticists, biologists, and practicing physicians, the Senate Subcommittee of Separation of Powers concluded in a report published in 1981 the following: "The testimony of these witnesses and the voluminous submissions received by the Subcommittee points to a clear conclusion: the life of a human being begins at conception." (U.S. Senate Subcommittee on Separation of Powers, Report on The Human Life Bill -- S. 158 (Dec. 1981) - Dr. Hymie Gordon (Mayo Clinic): "By all criteria of modern molecular biology, life is present from the moment of conception." - Dr. Micheline Matthews-Roth (Harvard University Medical School): "It is scientifically correct to say that an individual human life begins at conception." - Dr. Alfred Bongioanni (University of Pennsylvania): "I have learned from my earliest medical education that human life begins at the time of conception." - Dr. Jerome LeJeune, "the Father of Modern Genetics" (University of Descartes, Paris): "To accept the fact that after fertilization has taken place a new human has come into being is no longer a matter of taste or opinion . . . it is plain experimental evidence."

Dr. Kevin Winslow discusses oocyte cryopreservation

Dr. Andreyko summarizes IVF with egg (oocyte) donation and gestational surrogacy.

How the Body Works Immature Female Sex Cells Immature sex cells, or oogonia, in the ovary divide by mitosis to produce oocytes. Before ovulation, one oocyte divides by meiosis to produce a secondary oocyte with half the full number of chromosomes, and a polar body. At fertilization, further divisions result in the production of the mature ovum and three superfluous polar bodies.