Discover

'Tissue culture' refers to the growth of tissues and/or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth media, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, while the more specific term plant tissue culture is used for plants.

Contents

Historical usage

Modern usage

References

See also

External links

Historical usage

In 1885 Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture.^[1]
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog nerve cell processes in a medium of clotted lymph.

Modern usage

Main articles: cell culture

In modern usage, "tissue culture" generally refers to the growth of eukaryotic cells ''in vitro''. It is often used interchangeably with '''cell culture''' to specifically describe the ''in vitro'' culturing of mammalian cells.
However, "tissue culture" can also be used to refer to the culturing of tissue pieces, i.e. explant culture or whole organs, i.e. organ culture.

References

1. "Animals and alternatives in testing."

See also

★ Cell culture

★ Organ culture

External links

★ Splitting and Passaging Cells in Tissue Culture

★ www.research.umbc.edu/~jwolf/method5.htm - how-to guide for biology students

★ Plant Tissue Culture - way to "xerox" a plant

★ CELOS - a division of CELOS (Center for Agricultural Research in Suriname)