Discover

The 'murine leukemia virus' belongs to the gammaretroviral genus of the Retroviridae family of viruses, their hosts are vertebrates. It is a Type VI: positive sense ssRNA viruses that replicates through a DNA intermediate, reverse transcriptase.

Contents

Structure of Virus

Genome

Applications of MuLV

References

Structure of Virus

The virus particle consists of 65% protein, 35% lipid and 3% carbohydrate. As it is a type of retrovirus, it has a complex structure, consisting of a virus envelope which is studded with carbohydrate molecules (which originate from host cell membranes during virus exit from the host cell). The virus also has a spherical nucleocapsid which contains the nucleocapsoid. The virus is a spherical particle of approximately 90nm diameter.

Genome

The genome of the murine leukemia virus has been fully sequenced; it is a single stranded, linear, positive-sense RNA molecule of around 8000 nucleotides. The virus RNA molecule contains a 5' methylated cap structure and 3' poly-A tail. The genome codes for both structural and non-structural proteins (such as RNA-dependent DNA polymerase which is important during the reverse transcription stage).

Applications of MuLV

★ Gene therapy

★ Cancer studies - this virus can be used to study cancer mutagenesis.

References

★ http://www.virology.net/Big_Virology/BVretro.html

★ http://www.virologyj.com/content/1/1/14