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'Granulocyte-macrophage colony-stimulating factor', often abbreviated to 'GM-CSF', is a protein secreted by macrophages, T cells, mast cells, endothelial cells and fibroblasts.

Contents

Functions

Glycosylation

Clinical significance

Leukine

Controversy

See also

References

External links

Functions

GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exit the circulation and migrate into tissue, whereupon they mature into macrophages. It is thus part of the immune/inflammatory cascade, by which activation of a small number of macrophages can rapidly lead to an increase in their numbers, a process crucial for fighting infection.

Glycosylation

Human granulocyte macrophage colony-stimulating factor is glycosylated in its mature form. The glycosylation sites are reported to be at amino acid residues 23 (leucine), 27 (asparagine), and 39 (glutamic acid) (see US Patent No. 5,073,627).^[1]

Clinical significance

GM-CSF is also known as molgramostim or, when the protein is expressed in yeast cells, 'sargramostim' ('Leukine®').
GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy. It has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising but GM-CSF is not presently FDA-approved for this purpose.

Leukine

''Leukine'' is the trade name of sargramostim manufactured by Berlex Laboratories, a subsidiary of Schering AG. Its use was approved by U.S. Food and Drug Administration for acceleration of white blood cell recovery following autologous bone marrow transplantation in patients with non-Hodgkin's lymphoma, acute lymphocytic leukemia, or Hodgkin's disease in March 1991.^[2] In November 1996, the FDA also approved sargramostim for treatment of fungal infections and replenishment of white blood cells following chemotherapy.^[3]

Controversy

Berlex funded a study that ran in the May 26 2005 issue of the ''New England Journal of Medicine'' which concluded that, GM-CSF did produce significantly more remissions in Crohn's disease than those who received a placebo in the study, and it also decreased disease severity and improved quality of life.^[4]
The study's lead author, Joshua Korzenik of Harvard Medical School and Massachusetts General Hospital, is a paid consultant for Berlex , and co-inventor of the patent which is owned by Washington University.^[5] Korzenik created a "firewall" to protect the integrity of the study, consisting of two committees to review the study results and process as well and sending trial data to outside clinicians for review.

See also

★ Granulocyte macrophage colony-stimulating factor receptor

References

1. http://www.google.com/patents?vid=USPAT5073627&dq=5,073,627
2. [1] Details of Approved Claims by Line of Therapy, Approved Claims for 1991, FDA.gov
3. [2] Drugs Approved by the FDA, Drug Name: Leukine (sargramostim), CenterWatch.com
4. Sargramostim for active Crohn's disease, Korzenik J, Dieckgraefe B, Valentine J, Hausman D, Gilbert M, , , N Engl J Med, 2005
5. "Med school drug pushers: How scientists are selling out to drug companies", by David S. Bernstein, The Phoenix. Published April 12 2006. Accessed 8 Feb 2007.

External links

★ http://www.leukine.com/ - Official Leukine web site

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