
Deoxycytidine triphosphate
'Deoxycytidine triphosphate' ('dCTP') is a
cytidine nucleotide triphosphate that is used whenever
DNA is synthesized, such as in the
polymerase chain reaction.
e.g.:
A
chemical equation can be written that represents the process:
: (DNA)
n + dCTP ↔ (DNA)
n-C + PP
i
That is, dCTP has the PP
i (
pyrophosphate) cleaved off and the
dCMP is incorporated into the DNA strand at the 3' end.
Subsequent hydrolysis of the PP
i drives the equilibrium of the reaction toward the right side, ie. incorporation of the nucleotide in the growing DNA chain.
It is stored at -20 degrees
Celsius.
See also
★
DNA Replication