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'Day of Atonement' may refer to:

★ Yom Kippur, the Jewish Day of Atonement

★ Day of Atonement (Nation of Islam), a national day established in 1995 by the Nation of Islam

★ Investigative Judgment, the antitypical Day of Atonement according to Seventh-day Adventist teaching.

★ Day of Atonement (Christian holiday), a day of fasting kept by a minority of Christians on the same day as Yom Kippur

★ ''Day of Atonement (play)'', a play by Samson Raphaelson

★ ''Day of Atonement (novel)'', a novel by Jay Rayner